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Kerkar, Savita
- Population Dynamics of Bacterioplanktonic Component Associated with the Phytoplankton Biomass in Kongsfjorden, an Arctic Fjord
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Authors
Affiliations
1 National Centre for Antarctic and Ocean Research, Head Land Sada, Vasco-da-Gama 403 804, IN
2 Department of Biotechnology, Goa University, Taliegao Plateau 403 206, IN
1 National Centre for Antarctic and Ocean Research, Head Land Sada, Vasco-da-Gama 403 804, IN
2 Department of Biotechnology, Goa University, Taliegao Plateau 403 206, IN
Source
Current Science, Vol 115, No 9 (2018), Pagination: 1690-1694Abstract
Temporal variation (June and October 2012) in bacterial and phytoplankton communities of Kongsfjorden was studied using 16S rRNA gene clone libraries and marker pigments respectively. Proteobacteria was the dominant phyla in Kongsfjorden with Gammaproteobacteria (42%) and Alphaproteobacteria (84%) dominating in June and October, respectively. Retrieval of sequences affiliated to Verrucomicrobia, Gammaproteobacteria and Bacteroidetes in June corresponded with high autotrophic biomass (Chl α, 33 ng l–1) whereas abundance of SAR 11 coincided with decrease in the intensity of autotrophic biomass (Chl α, 24 ng l–1) in October. Thus, the distribution of bacterioplankton community varied with change in phytoplankton composition indicating a significant coupling between these two groups in the fjord water.Keywords
Arctic, Bacterial Diversity, Kongsfjorden, Phytoplankton Pigments.Full Text
References
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- Enhancing Biosurfactant Production by Hypersaline Bacillus amyloliquefaciens SK27 using Response Surface Methodology and Genetic Algorithm
Abstract Views :232 |
PDF Views:70
Authors
Affiliations
1 Department of Biotechnology, Goa University, Goa 403 206, IN
1 Department of Biotechnology, Goa University, Goa 403 206, IN
Source
Current Science, Vol 117, No 5 (2019), Pagination: 847-852Abstract
The use of biosurfactants has been limited because of their low yield and high production cost. A central composite design was used to study the interactive effect of sucrose, yeast extract and sodium chloride which were the most influencing variables. Response surface analysis showed that the quadratic model with R2 value of 0.9983 was fit for biosurfactant production. When genetic algorithm was used for maximization, the optimal activity (oil displacement zone) was found close to that obtained by response surface methodology, both of which were close to the predicted value. Biosurfactant production was enhanced by 1.2- fold using these approaches.Keywords
Bacillus amyloliquefaciens, Biosurfactants, Central Composite Design, Genetic Algorithm, Response Surface Methodology.Full Text
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- Fernandes, M. S. and Kerkar, S., Enhancing the anti-tyrosinase activity of a hypersaline Kitasatospora sp. SBSK430 by optimizing the medium components. Curr. Sci., 2019, 116(4), 649–653; doi:10.18520/cs/v116/i4/649-653.
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- Enhancing the Anti-Tyrosinase Activity of a Hypersaline Kitasatospora Sp. Sbsk430 by Optimizing the Medium Components
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PDF Views:66
Authors
Affiliations
1 Department of Biotechnology, Goa University, Goa 403 206, IN
1 Department of Biotechnology, Goa University, Goa 403 206, IN
Source
Current Science, Vol 116, No 4 (2019), Pagination: 649-653Abstract
Tyrosinase inhibitors from natural resources have been gaining importance in pharmaceutical and horticultural applications. A full factorial central composite design was used to study the interactive effect of three variables, i.e. D-mannitol, yeast extract and sodium chloride of the fermentation medium for maximizing anti-tyrosinase activity (75.5%) of a hypersaline actinobacteria, Kitasatospora sp. SBSK430. A quadratic model was found to fit the anti-tyrosinase activity (R2 = 0.948). Response surface analysis revealed that the optimum values of the medium components were 15 g/l D-mannitol, 5.6 g/l yeast extract and 1.2 g/l sodium chloride. Tyrosinase inhibition activity was enhanced 1.1-fold, using this approach.Keywords
Actinobacteria, Anti-Tyrosinase, Fermentation Medium, Hypersaline, Kitasatospora sp.Full Text
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